This paper presents a method for obtaining dry extract from wild rose hips (Rosa platyacantha) using percolation. Ethyl alcohol at a concentration of 40% was used as an extractant. Three days settling at a temperature of 10 ± 5 °C to purify the extraction and filters with a pore diameter of 1.0 цт, 0.5 цт, 0.65 / 0.45 цт for threefold filtration were used. Drying of the extract was carried out using spray drying. The finished product meets the requirements of the State Pharmacopoeia of the Republic of Kazakhstan.
Relevance. Briar (Rosa) of the Rosaceae family (Rosaceae) is one of the most widely used medicinal plants in both official and traditional medicine, due to its rich chemical composition. Rose hips contain a large amount of vitamins and other biologically active substances: vitamin C, vitamin B1, vitamin B2, vitamin P, vitamin PP, carotene, tocopherols, flavonoids, lipids, organic acids, tannins, essential oils, carbohydrates, etc. [1, 2]. This genus includes 366 officially recognized species, 76 species of which grow on the territory of the Republic of Kazakhstan [3].
One of the many representatives of the genus dog rose, which is of scientific and practical interest, is the rose hip (Rosa platyacantha Schrenk). Scientists of the School of Pharmacy of the National Medical University are conducting research on obtaining pharmacopoeial herbal substances from various parts of the plant under study for the production of perfumes and cosmetics.
The aim of this work is to develop a technology for obtaining from the rose hips of a dry extract that meets the pharmacopoeial criteria.
Materials and methods. Description of the production process. In the production of the dry extract, the following ingredients are used: rose hips, harvested in accordance with the instructions for the preparation and storage of raw materials in accordance with the requirements of "Good practice of cultivation and collection of medicinal plants WHO" [4], purified water and ethyl alcohol 96% of pharmacopoeial quality.
The technological process consists of the following operations: preparation of raw materials, obtaining the extraction, cleaning, drying, packaging and labeling. The flow chart is shown in Figure 1.
Figure 1 Technological scheme of the production of dry extract from the rose hips
The calculated number of rose hips was crushed in a mill to a size of 0.5-3 mm, the crushed raw material was loaded into the maceration tank and filled with the calculated amount of extractant, ethyl alcohol 40%, soaked for 4 hours at a temperature not higher than 25°С. After the specified time, the swollen raw material was loaded into the percolator on a false bottom, filled with the extractant to the mirror and held for 24 hours at a temperature not higher than 25°С.
Then percolation was started at a speed of 1/24 rev/h (the ratio of raw material and extractant is 1:10). Then the drains were combined, the meal was pressed. The obtained semi-product was settled at a temperature of 10 ± 5T within 48 hours. The pooled extract was separated from the ballast mass, subjected to threestage filtration (pore size of filters 1.0 ^m; 0.5 ^m; 0.65 / 0.45 ^m), and dried using a spray dryer. The resulting powder was packaged in plastic bags, which were sealed; then they were labeled in accordance with the requirements of order No. 227 of 04/16/2015 [5] and packed in cardboard packs.
Results and discussion. Table 1 presents the technological parameters studied in the process of production of the dry extract from rose hips.
Table 1 -
Process stages |
Parameters |
Regulated norms |
The number of samplings in one series |
Stage 1 |
Feed quality |
In accordance with NTD: tech. |
1 |
Preparation of the |
regul. |
||
extractant |
Mass (volume) of the raw materials used |
± 50 mg |
1 |
Mixing time |
30 min |
1 |
|
Mixing speed |
15 rev/min |
Every 10 minutes |
|
Ethanol concentration |
39 41 % |
3 points |
|
The amount of ethyl alcohol |
± 50 mg |
1 |
|
Stage 2 Preparation of |
Quality of the raw materials |
In accordance with NTD: tech. |
1 |
MPRM |
Mass of the raw materials used |
regul. ± 50 mg |
1 |
Stage 3: Obtaining Extraction from MPRM |
Extraction temperature |
from 20 to 25°С |
Every 10 minutes |
Extraction time Soaking |
4 hours |
1 |
|
Infusion |
24 hours |
||
Percolation |
1/24 rev/h |
||
Identification of biologically active substances (BAS) |
In accordance with the JV |
3 points |
|
Ethyl alcohol concentration |
In accordance with the JV |
3 points |
|
Quantitation |
In accordance with the JV |
3 points |
|
Stage 4 Purification |
Settling temperature |
10±5°C |
Every 30 |
of the
for the
of the dry extract from rose hips
(Settling, Filtration) |
minutes |
||
Settling time |
48 hours |
1 |
|
Filter pore size |
1.0 |Ж 0.5 ^m; 0.65/0.45 um |
1 |
|
Quality of the semi-product |
In accordance with the JV |
3 points |
|
Stage 5 Drying |
Temperature |
In accordance with the regulations |
Every 10 minutes |
Pressure |
In accordance with the regulations |
Every 10 minutes |
|
Air velocity |
In accordance with the regulations |
Every 10 minutes |
|
Stage 6 Preparation of packaging materials |
Quality of packaging materials |
In accordance with the JV |
3 samples |
Microbiological purity |
In accordance with the JV |
3 samples |
|
Stage 7 Dispensing, marking and packing of finished products |
Packing weight |
50 ± 0.05 g In accordance with NTD: tech. regul. JV |
1 |
Completeness |
In accordance with the JV |
1 |
|
Marking quality |
In accordance with the JV |
1 |
On the basis of the developed plan for the production process critical stages were determined and optimal technological parameters were selected, samplings were compiled and tested. Regulatory documentation for assessing the quality of raw materials, semi-finished and finished products was predeveloped. The resulting finished product meets the requirements of the project IDA and the State Pharmacopoeia of the Republic of Kazakhstan [6-8].
Findings. The optimal technology has been developed and tested for obtaining dry extract from the wild rose hips. Regulated norms of technological parameters for critical technological stages have been determined. The quality of the finished product meets the requirements of the State Pharmacopoeia of the Republic of Kazakhstan. On the basis of the developed technology, laboratory regulations have been created for the production of dry extract from rose hips.
REFERENCES
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