Hydrogels and cryogels based on fmoc-phephe nanotubes as efficient carrier of active pharmaceutical ingredient

The drug delivery systems(DDS) fabricated via self-assembly of biocompatible nonalergic components is of great importance for treatment of chronic wounds and diseases. The benefit of the DDS composed of short sequence peptide derivative compare to polymeric DDS is absence of immunogenic response, formation of the hydrogel based on nanofibers quickly upon injection of solution of pharmaceutical active ingredient to a low molecular weight gelator with pH changing, Fmoc(Phe)3 hydrogels and other hydrogels were used for delivery of Desamethsone [1-5].

In this study inclusion of biologically active substances during the hydrogel formation via selfassembly of Fmoc(Phe)2 in situ was studied. We have shown that novel hydrazides and corresponding hydrazones containing piperidine or harmine heterocycle are very perspective pharmacutical substances[6-7].

Cryogels and hydrogels via self-organization Fmoc-(Phe)2 were prepared [5]. The kinetics of desorption of the substance a 3,4-dihydroxy-benzylidenhydrazide 2-methyl-3-(N-piperidinyl)propionic acid in 0.01M phosphate buffer was examined using UV spectrophotometery.

Thus, the maximum concentration of the biologically active substance was achieved within 5 hours at pH 5.8 and 7 and were equaled to 1.6 and 1.9 x 10-5 Mol/L, respectively. DDS of cryogels Fmoc(Phe)2 revealed the equilibrium concentration of desorbed substance of 3.11 and 3.47 x 10-5 Mol/L at pH 5.8 and 7.0, respectively. Three-fold increase of the 3,4-dihydroxy-benzylidenhydrazide 2-methyl- 3-(N-piperidinyl)propionic acid desorption from cryogel compare to the hydrogel with the same loading capacity of the drug can be attributed to high porosity of the scaffold.

The cryogel has a large surface area, from which the desorption of the substance takes place faster. It can be concluded that DDS of Fmoc(Phe)2, or other peptides can be loaded with various concentration of drug and one can also modulated the rate of release by change of morphology. Usage of circular dichroism allows to estimate the mechanism of self-organization upon addition of the drug and to evaluate a relative fraction of the drug that was not entrapped to the gel structure.


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Year: 2021
City: Shymkent
Category: Medicine