L-arginine as a biologically active additive in therapeutic and prophylactic dental pastes on a gel basis

Currently, the production of toothpaste is a complex production process that has undergone a long period of research and modernization. The range of application of toothpastes is quite extensive. For example, they are used not only as hygienic means for removing plaque, but also in the complex treatment of oral diseases. Depending on the addition of biological active substances (BAS), they may have therapeutic and prophylactic properties. In this paper, as a BAS, we examine the amino acid L-Arginine.

L-Arginine is a building material of proteins and is part of many of them. This conditionally replaceable amino acid, which has the following properties: antibacterial, anti-inflammatory, regenerating, antioxidant, and others. In addition, it has a positive effect on the work of the nervous and cardiovascular systems, affects the functioning of the liver, affecting its detoxification function. When applied externally, this amino acid provides skin elasticity and stimulates the defense systems of our body; it easily penetrates the skin and mucous membrane. In the normal functioning of the body, L-Arginine is produced in sufficient quantities by the liver. But this process can slow down or stop, due to violations against the background of various diseases and as a result of aging of the body.[3]. In this regard, in this work, we propose to introduce L-Arginine into the composition of toothpaste. With the introduction of it as a BAS, it acquires a high therapeutic effect and has a beneficial effect on the gum tissue, accelerating the healing process. Therefore, it is possible to use this toothpaste for stomatitis, gingivitis and other diseases of the oral cavity.[1,2].

Along with this, we studied the condensed grape juice (bekmes).

Grape juice has been used for a long time, and is a valuable product for many diseases. In medicine, there is a special therapy - ampelotherapy - viticulture.

Grape juice reduces cholesterol in the blood and normalizes blood pressure, increases physical and mental activity, rejuvenates tissues, and opposes a variety of viruses, for example, the herpes simplex virus.[6].

Bekmes is a concentrated evaporated grape juice, which we consider not only as a flavor in the toothpaste, but also as an antioxidant.

Due to the rich chemical composition determines a wide range of bekmes. More than 150 biologically active substances are found in it and are an alternative to honey.[5].

Based on literary sources, we took gel as the basis for this toothpaste.

The main components of the paste are: sodium alginate as a gel-forming substance, silicon dioxide as an abrasive, sodium lauryl sulfate as a foaming agent, purified water and glycerin as binders, citric acid as a preservative, bekmes as a flavoring agent and an antioxidant, a mixture of essential oils (peppermint oil and grape oil seed) as a fragrance and L- Arginine as a BAS.

The work was carried out in the laboratory of the Department of Drug Technology of SKMA using the box of abacterial air БАВп-01-«Ламинар-С.»-1,2 (220.120.04).

The method of preparation is as follows: sodium alginate was dissolved in the calculated amount of purified water and left to swell. Glycerin and purified water were added to the mixture of powders consisting of silicon dioxide, sodium lauryl sulfate, citric acid. Next, we put the swollen sodium alginate in a mushy mass and mixed until homogeneous. In parallel with this, an aqueous solution of L-arginine was prepared, then added to the resulting mixture.

To the mixture obtained, a calculated amount of condensed grape juice was added, which was received in advance at the Department of Drug Technology from Tayfi pink grapes. Juice was obtained and evaporated using a rotary evaporator RVIKA 10 digitalV.[4].

Last but not least, with constant stirring, we added a mixture of essential oils.

The final stages of the preparation of this toothpaste were homogenization and standardization.

In accordance with the Interstate Standard ГОСТ 29188.2-91 «Изделия косметические. Метод определения водородного показателя рН», the pH value of this toothpaste was determined. We used ionomer И-160 МИ, intended for potentiometric measurement of the activity of hydrogen ions (pH). For the analysis, a water suspension was prepared with a mass fraction of 25% according to the Interstate Standard ГОСТ 7983-99 «Пасты зубные. Общие технические условия». The suspension was placed in a glass with a capacity of 50 cm3, the ends of the electrodes were immersed in the test liquid.

According to ГОСТ 29188.2-91 «Пасты зубные. Общие технические условия», for the final result of the analysis, the arithmetic average of the results of two parallel determinations is taken, the allowable discrepancy between them should not exceed 0.1 pH units. The data analysis is shown below in Table 1.

Table 1.


In the test sample



5,5 – 10,5





Conclusion: In this way, in the process of studying the composition of toothpastes, we proposed the composition and technology of preparing gel-based toothpaste with therapeutic and prophylactic properties. The analysis was carried out according to the general specifications for toothpastes. Research in this direction is ongoing.


  1. JanBernard, DanMcGeehan, ToothpasteBeforetheStoreLibraryBinding – January 1, 2012.
  2. Saqib Ali, Robert Hill, David Gillam, Abrasivity of Bioactive Glass toothpaste for treating Dentine Hypersensitivity Paperback – October 1, 2012.
  3. Tsai P., Weaver J., Cao G.et al. L-Arginine regulates neuronal nitric oxide synthesis production of superoxide and hydrogen peroxide//Biochem. Pharmacol. – 2005. – Vol.69. – P.971-979.
  4. Pavia D.L. Introdaction to Organic Laboratory Techniques: A Small-scale Approach. – Cengage Learning, 2005. – C. 643.
  5. Липкан Г.Н. Применения плодово-ягодных растений в медицине. К.: Наукова думка, 1985. – С. 43.
Year: 2018
City: Shymkent
Category: Medicine