The air environment is one of the most common factors in the transmission of airborne infections, both bacterial and viral [1]. The problem of bacterial air pollution is especially acute in organized collectives, for example, in schools.
The microflora of the air of organized collectives like schools, preschool institutions has become the subject of growing concern at the present time. It should be noted that pupils stay at school for the most part of active time, sometimes up to 7-8 hours [2].
The purpose of this study was to examine the microflora of the classrooms of the Educational High School "Murager", a special issue was the evaluation of the effectiveness of the bactericidal recirculator "AEREX-constants" on the classroom’s microflora
Object and methods of research. This international scientific project is carried out in the framework of academic mobility in cooperation with students Mustafina Assem (K S M U), Demirtola (Baskent University, Turkey), Odul Tani (Trakya University, Turkey) and Upasheva Amina (student of 11th grade EHS "Murager")
The microflora of air was investigated in the classrooms (203,213) of the Murager school in the winter (January) and the summer (June). The effectiveness of the bacteriocidal recirculator "AEREX- constants" was tested in the study room 213, the classroom 203 served as a control, without the bacteriocidal recirculator.
Microbiological investigations were performed at the Department of Microbiology of KSMU. The microflora of the air in the study rooms was determined by the Koch sedimentation method [3].The following indicators were studied: the most probable number (MPN), hemolytic microorganisms, staphylococci, fungi in 1 m3 of air.
Result:The microflora of the air in the classroom was significantly different depending on the season of the year. In the summer ,initially before classes the MPN was 953 CFU / m3, while in the winter the same indicator was 2256 CFU / m3 and estimated as moderately polluted. After 1 lesson in the winter, the air MPN increased 2 times from 2256 CFU / m3 to 4469 CFU / m3, the air from the moderately polluted became contaminated. Despite the fact that in the summer the MPN after the first lesson increased by 1.6 times (from 953 CFU / m3 to 1574 CFU m3) the air continued to remain relatively clean. The number of hemolytic microorganisms before and after the lesson was also higher in winter (967 CFU / m3 ,1756 CFU / m3 ) than in summer (353 CFU/m3 ,409 CFU / m3). The number of staphylococci before and after the lesson was more than 2 times higher in winter (856 CFU/m3,1840 CFU/m3 ) than in summer (417 CFU/m3, 677 CFU/m3) Fungi were detected only in winter 411 CFU/m3 before classes, and 675 CFU/m3 after 1 classes.
Under the influence of a bactericidal recirculator in the class room 213, MPN was elevated at 1.65, without it (203 rooms), this indicator increased by 1.45 times. In the analysis of staphylococci obtained similar results: in a room with a recirculators the amount of staphylococci increases 1,7 times without it 1.6 times. The number of microorganisms after the first lesson in the testing and control rooms increased approximately the same.
Hemolytic microorganisms after 1 lesson increased by 1.5 times in the control room and 1.15 times with recirculator. The recirculator acts slightly on hemolytic streptococci, the resistance of which in the external environment is lower , in comparison with other microorganisms. This assumption requires more detailed study.
Conclusion:The microflora of classrooms depends on the season of the year. In the winter, the indicator microorganisms of air were detected 2 or more times higher and aggressive than in the summer. Fungi were tested only in winter.
The ultraviolet bactericidal recirculator "AEREX constant did not show the expected and advertised effectiveness.
References
- Noakes C.J. Modelling the transmission of airborne infections in enclosed spaces // Noakes C.J., Beggs C.B, Sleigh P.A, Kerr K.G. Epidemiology and Infection.- 2006. -№5.-P. 1082-1091
- Rana M. Badri Identification and characterization of air bacteria from some school of Baghdad city// Rana M. Badri, Rana R. Alani, Sura Sami Hassan Mesopotamia environmental journal.- 2016.-Vol.2.- №4.- P. 9-13.
- Сбойчаков В.Б. Санитарная микробиология - М.: ГЭОТАР