Summary
The objective of our study were to analyze the trace and macro elements composition on an aerial part of Agrimonia asiatica. By using atomic absorption spectrophotometry method identified 17 chemical elements. Among that elements, the main role plays the macro element P, and trace elements like V, Cd, Be, Ni, Mo, Cu, Fe, Zn, Mn. Also the acceptable amount of Pb, Sn are normal. The most abundant among the essential elements are P, Al, Zn, Mn, Cu, Ni, whereas Pb was predominant among the tested non-essential elements followed by Sr
and Sn. The results showed that the concentration transfer differed significantly among all elements. Elements such Sb and Se absent in plant sample material.
Keywords:Agrimonia asiatica,mineral elements, atomic absorption spectrophotometry, macro element, trace elements.
Introduction. The study of elemental composition of plants are urgent for entirely characterization of location of chemical elements in natural origins, as well as the plant is the most target of biology interaction. In practical point of view, data on chemical content of eatable and medicinal plants is important, as balanced agent for people and to treat illnesses causing with lack of elements. The main attention delivered also for heavy metals, and toxic elements to regulate their concentration. In plants, it is determined more than 80 chemical elements and we classify them into macro and trace elements. These classification is undergo by the principle of quantitative content of elements in plant tissues. Regulations relating to metals have been established for manyproducts of vegetables origin in many countries to protect theconsumer from the harmful effect of these chemicals. Concerningthe European Union, there are no regulations specifying theacceptable content of metals in tea, in contrast to the other countries, especially extra European countries, which for some metalshave already developed their own national guidelines to pledgesafety and quality of this product [1]. The extraction of trace metals, suchas Mn, Zn and Al, was also relatively high [2].
Materials and methods. The object of our research is an aerial part of the plant Agrimonia asiatica, which is collected at blossoming period and dried at room temperature. Crushed plant materials used to conduct dry mineralization [3]. Obtained samples then used to atomic absorption spectroscopy model MGA-1000 by LUMEX for determining elements.
Statistical analysis. To compare chemical levels between the analyzed samples the statistical test was applied. If the assumption of normality could besustained, a classical ANOVA test was applied.
Results and discussion. In table 1 is given the obtained results. The results of the measurements are defined by the next formula X ± Ax;
|
Chemical elements |
Content of mineral elements calculated on ash, mg/kg |
LAC* (SanLaN*2.3.2.560-96) |
|
|
Tea |
Beverages |
||
|
Macro elements |
|||
|
P |
2506,75±501,35 |
- |
- |
|
Trace elements |
|||
|
Al |
1654,25±595,53 |
- |
- |
|
Zn |
40,54±10,54 |
- |
10,0 |
|
Mn |
28,6±10,3 |
- |
- |
|
Sr |
21,18±6,35 |
- |
- |
|
Fe |
7,74±2,01 |
- |
- |
|
Sn |
2,24±1,12 |
- |
- |
|
Cu |
2,05±0,41 |
- |
- |
|
Ni |
1,93±0,48 |
- |
- |
|
Pb |
1,47±0,001 |
- |
- |
|
Cr |
1,03±0,26 |
0,03 |
1,0 |
|
V |
0,8±0,2 |
- |
- |
|
Cd |
0,37±0,09 |
10,0 |
0,3 |
|
Cb |
0,1±0,02 |
- |
- |
27
|
Be |
0,00475±0,00123 |
- |
- |
|---|---|---|---|
|
Sb |
absent |
- |
- |
|
Se |
absent |
- |
- |
LAC* - limit acceptable coefficiency SanLaN* - sanitary limit and norm
Conclusion. In this study, concentrations of trace elements and macro elements in Agrimonia asiatica herb were identified. Generally, the most abundant element among the macro elements was P, whereas the Mn, Fe and Zn content was prominent among the trace elements tested, followed by Al. The extraction of trace elements, such as Mn, Zn and Al, was also relatively high.
References
Grazia Barone, Roberto Giacominelli-Stuffler, Maria M. Storelli, «Evaluation of trace metal and polychlorinated biphenyl levels in tea brands of different origin commercialized in Italy», Food and Chemical Toxicology 87 (2016) 113-119
Malgorzata Dambiec, Ludmila Polechonska, AgnieszkaKlink, «Levels of essential and non-essential elements in black teas commercialised in Poland and their transfer to tea infusion» Journal of Food Composition and Analysis 31 (2013) 62-66
Method M 04-64-2010 "Food and raw materials for food. Feed and raw materials for their production. Method for measuring the mass fraction of cadmium, lead, arsenic, mercury, chromium, tin by atomic absorption spectroscopy using an atomic absorption spectrometer with electrothermal atomization of the modifications MGA-915, MGA-915M, MGA-915MD, St. Petersburg, 2010