The development of methods of qualitative and quantitative analysis of l-arginine in medicinal preparations


This article describes a technique of identification and quantification of arginine in the composition of medicinal preparations.

Keywords: arginine, an amino acid, a drug.

Purpose of the study: looking for optimal methods of establishing quantitative and qualitative analysis of arginine in medicinal preparations one of the various sources of literature.

Research: Arginine is a conditionally replaceable amino acid of the human body. Potential arginine when it is used as an active ingredient is very high - it affects the biological basis for the functioning of the human organism. Arginin is one of the most powerful antagonists comrade various tumors in human skin (tumors, papillomas, warts, etc.), as well as a means to stimulate regeneration of the skin during herfirst injury (trauma, burns). Therefore, it is widely used in cosmetology. [1] It is widely used in medicines and we need to choose the best option among the available methods of quantitative and qualitative analysis of arginine in various preparations. The work is based on the study of methods for analyzing the content of arginine, proposed by various sources. We have chosen the official methods proposed by the State Pharmacopoeia of the Republic of Kazakhstan:

  1. Ninhydrin reaction method is a qualitative determination of arginine and is used for visual identification the chromatograms (thin paper layer), as well as for determining the concentration of arginine colorimetric method according to the color intensity of the reaction product. When added to a solution of arginine alcoholic solution of ninhydrin with heating appears blue-violet color
  2. The major and best-known method for the quantitative analysis method is arginine anija titers using 0.1 M hydrochloric acid solution as a titrant adding methylene red indicator. 1 ml 0.1 M HCl corresponds to 17.42 mg of arginine.
  3. Sakaguchi reaction: 10% NaOH solution is added to an alcohol solution of a-naphthol. Stir, add 1-2 drops of sodium hypochlorite and mix again. Rose-red color develops. [2]

In addition, other sources offer alternative methods for the analysis of arginine.

The analysis of amino acids has also recently been carried out by the thin-layer method chromatography. The authors found out that separation and the greatest differences in arginine mobility were obtained using the following solvent mixtures: in the first direction the heptane-dichloroethane-acetic acid mixture (20:30:30); in the second (perpendicular) direction, the butanol-acetic acid-water mixture (80:20:20). The mobility factor Rf, obtained under conditions of an ascending two-dimensional TLC on Armstrong plates for arginine, is 0.12.

The disadvantages of this method are: the instability of the connection with arginine, as well as the long waiting time of the result (about 3 days). However, this method is very accurate and does not require expensive equipment. At present, thin-layer chromatography is used for preliminary analysis. [3]

In comparison with the method described above, the paper chromatography method is more accessible. During the work, the same development systems as for TLC are used. The value of Rf when using F4 paper for arginine in the BUV system of 4:1:2 is 0.04 [4]

Conclusion: We studied and mastered a variety of methods of identifying and study of L-arginine in substances. It is established that the methodology proposed by the official documentation (Pharmacopoeia of Republic of Kazakhstan) is the most convenient and effective. Thus, the goals have been realized, the result has a serious practical value. Work in this direction continues.


List of used literature

  1. Tapiero, H.; et al. (November 2002). "L-Arginine". Biomedicine and Pharmacotherapy 56 (9): 439445
  2. State Pharmacopoeia of the Republic of Kazakhstan v. 2, p. 110
  3. VN Maystrenko, RR Ilyasova, F. Kh. Kudasheva et al. Quantitative analysis of a- amino acids in the urine of patients with neurosurgical by thin-layer chromatography plates "Armsorb" //Bulletin of the Bashkir University. 2008. T. 13. No. 2. P. 265-269
  4. V.F. Selemenev, V.Yu. Khokhlov, O.V. Bobreshova and others. Physicochemical bases of sorption and membrane methods of separation and separation of amino acids. -In oronezh. 2001. 300 s
Year: 2017
City: Shymkent
Category: Medicine