The genus Tagetes L., Asteraceae family (Marigolds) consists of about 59 species of annual and perennial plants [1]. It have been used as sources of carotenoids (xantofills), helenien and essential oil. In folk medicine Tagetes L. have been widely used for treat headaches, fevers, colic, abdominal pain and infectional desises, as a tonic, as natural insecticides and fungicides [2]. Marigolds contain more than one hundred bioactive compounds, such as carotenoids, flavonoids and other phenolic derivates, essential oil, aminoacids, organic acids. Phenolic derivates are a large group of natural bioactive compounds. They have strong antioxidant activities, antimicrobial, anti inflammatory and hepatoprotective properties. The main phenolic derivates of the genus Tagetes L. are flavonoids and hydroxycinnamic acids [3].
The aim of our work is the study of the marigolds flowers phenolic composition.
Materials and methods. As plant material we used the dry inflorescences of marigold (T. patula nana L. var. "Goldkopfen''). Phenolic compounds were extracted by ethanol. This extract was studied on “Specord-200 Analytic Jena UV-vis” and —Shimadzu HPLC-system, ser.20”. As standarts we used coffeic acid, chlorogenic acid, quercetin, rutin, and apigenin.
Results. We identified 5 flavonoids and 2 hydroxycinnamic acids in inflorescences of T. patula nana L. var. —Goldkopfen”. Total content of flavonoids is 4,85±0,17%. The plant material contained chlorogenic acid (0,1255±0,0019%), caffeic acid (0,0041±0,0001%), patuletin, patulitrin, quercetin (0,7050±0,0106%), apigenin (0,0187±0,0003%) and rutin (0,0402±0,0006%).
Conclusion. We study T. patula nana L. var. ''Goldkopfen'' inflorescences phenolic composition by spectrophotometry and HPLC. We identified chlorogenic acid, caffeic acid and flavonoids quercetin, apigenin, patuletin, patulitrin and rutin. The main phenolic compounds are chlorogenic acid, patuletin and quercetin. Inflorescences of marigold are the perspective plant material for new drugs.
References
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