The study of periodontal pocket microflora in complex treatment with essentialoils (experimental study)

RESUME

The leading organisms in the etiology of periodontitis are anaerobes and some other aerobic microorganisms such as Streptococci, staphylococci, enterococci and etc., therefore, one of the main stay of successful treatment of this pathology in the oral cavity is the suppression of pyogenic microflora. The traditional usage of antimicrobial medicines is a complex treatment and doesn't provide long-term and sufficient effect. It seems appropriate to conduct more research for a new and more adequate methods of eradicating microbial agents that trigger an acute and chronic periodontal diseases.

In this regard, ultraviolet radiation in combination with Epherol (ie. 20% alcoholic solution of essential oil of wormwood smooth), developed by Phytochemistry, acorporation in the Ministry of education, department of science, Republic of Kazakhstan is more relevant.

Keywords: periodontitis, complex treatment, essential oils, microbial agents.

Introduction: The important role of immune response and microbial factors in the development of periodontal diseases is presented at the present stage.As a rule, the leading organisms in the etiology of periodontitis are associations of aerobic microorganisms such as Streptococci, staphylococci, enterococci, andanaerobes such as bacteroides and actinomyces,as well as other microorganismsplay a leading role in the etiology of paradonts. Therefore, one of the main stay of successful treatment of this pathology in the oral cavity is the suppression of pyogenic microflora.Here, the traditional usage of antimicrobial medicines is a complex treatment and doesn't provide long-term and sufficient effect. It seems appropriate to conduct more research for a new and more adequate methods of eradicating microbial agents that trigger an acute and chronic periodontal diseases.

In this regard, ultraviolet radiation in combination with Epherol (i.e. 20% alcoholic solution of essential oil of wormwood smooth), developed by Phytochemistry, a corporation in the Ministry of education, department of science, Republic of Kazakhstan is more relevant.

The aim of this work was to study the sensitivity of the isolated microorganisms from the periodontal pocket to ultraviolet radiation and Epherol in vitro study. You can also identify the bactericidal activity of some essential oils and their blends such as: essential oil of yarrow, essential oil of zizifor, the essential oil of cedar, essential oil of siberian fir.

Materials and methods: The experiment was conducted on test cultures like pyogenic Streptococcus (15 strains), S. aureus strains, enterococci strains, Candida albicans strains which were isolated from the periodontal pockets of patients with acute periodontitis. Sensitivity to laser radiation is determined by irradiation with ultraviolet lamp seeded with microorganisms on the surface of Petri dishes cultured with nutrient agar. This study used the following modes: output power grower with nozzles, was 1-3 milliwatts; exposure time was from 15 seconds to 10 minutes.

Results and discussion: 12 (Joule/sm2) is an average minimum inhibitory dose for aerobic flora, in particular to streptococci, enterococci, and staphylococci. The highest resistance to UV light appeared from fungi of the genus Candida (41, 7±1, 6 J/cm2). This mode was found to have maximum inhibition of pathogenic microorganisms. The study of the drug action of Epherol on the microflora of the periodontal pockets (streptococci, staphylococci, Enterococcus, E. coli, and Candida) had a bactericidal effect of preexposure with a microbial suspension with a given concentration from one billion microbial cells CFU/ml.

Conclusions: Ultraviolet radiation in the specified mode in combination with Epherol has a strong bactericidal effect on the microflora of the periodontal pocket and can be used in the treatment of patients with peridontitis at a dose of 0.4 J/sm2.

Year: 2015
City: Shymkent
Category: Medicine