Influence of the phytopreparation of the pink rhodiola to the functional condition of hepatocytes which were damaged by antitubercular preparations.

SUMMARY

At influence of anti-tubercular preparations there is seen decreasing resistance of biomembranes of hepatocytes to 70%, due to activation of per oxidation of lipids and a depression of AOS. Anti-radical action of PhPR depends on a dose, the most effectivedoses are at influence of anti-tubercular preparations of 2 ml/kg of weight due to 17 days treatment-and-preventetion actions of RDF-2 at intoxication drugs is caused by antioxidant action by influencing anti-tubercular preparations.

Key words: Pink rhodiola, phyto-preparations, resistance of biomembranes of hepatocytes, anti- tubercular preparations.

Purpose of Researching. Studying of influence of the phytopreparations of the pink rhodiola to the functional condition of hepatocytes which were damaged by antitubercular preparations.

Inclusion of PhPR in the treatment-and-preventetion mode with a dose 1,0ml/kg during 17 days will be reduced activity of AlAT(Alanin of amine transferase), AAsT(Amine aspartate transferase), PAWS which will be essential decreased their activity to 52%, 43,2%, 46,6% with comparison RIP group testifies. Activity of AlPh(ALKALINE PHOSPHATASE) decreased to 23,8%, and GGT(Gamma glutamit transpeptidaz) decreased to 4,1 times in comparison with uncured group of increasing RMG(Rezistention Membrane of Hepatocytes) was maded 126%. Using PhPR in a dose 2,0ml/kg of weight acutely decreased activity of AlAT, AAsT, LAP, AlPh and GGT to 65,6%, 63,6%, 65,3% , 39,1%, and 84% in comparison with uncured group, and decrease of the activity specified enzymes which are shown to 28,3%, 35,8%, 35,1% , 20% and 33,6% with the group which received PhPR in a dose of 1,0 ml/kg of weight. Resistance of membranes of hepatocytes increased more than triple and 44% reaches indicators of intact animals in comparison with uncured and second medical group (PhPR in a dose 1,0ml/kg).

Introduction. Development of new ways and means for treatment of acute damages of a liver is one of the actual problems of modern medicine. Now for treatment damage of a liver there are used representatives of various groups of medicines . However, using of these preparations do not always gives desirable therapeutic effect and there often observed collateral effects and complications[1]. High biological activity of the phytopreparation of a pink rhodiola (PhPR) is connected with existence in structure of weed flavonoids which free radicals are capable to inhibit [2,3].

Purpose of Researching. Studying of influence of the phytopreparations of the pink rhodiola to the functional condition of hepatocytes which were damaged by antitubercularpreparations.

Materials and methods of research. Researches are conducted on white rats(males) to whom during 14 days injected intratour antitubercular antibacterial drugs: rifampicin, isoniazid and pirazinamid (RIP). For an assessment of membranes tire-tread activity of PhPR on experimental animal the drug was injected to the stomach in a dose of 1,0 and 2,0 ml/kg of weight (3 doses before introduction of medicines and 14 doses together with RIP). The choice of this preparation is caused by existence of anti-toxic property of a preparation [4].

It served as the prerequisite for studying of efficiency of PhPR for prevention and treatment medicamentous complication at tuberculosis chemotherapy, in particular damage of a liver. As, it is known the treatment with antibacterial antitubercular preparations, there is most often shown the damage of hepatocytes due to toxic action of an isoniazid. rifampicin and pirazinamid [5].

There were studied some functional tests of hepatocytes for an assessment of pharmacological efficiency of PhPR , in particular defined indicators of a tsitoliz and holestaz of hepatocytes: AlAT, AAsT, LAP, AlPh and GGT. Results of an assessment of influence of PhPR on some indicators of functional tests of a tsitoliz and holestaz of hepatocytes are presented in table 1.

Table 1 - Influence of PhPR on functional activity of hepatocytes at injury of a liver by antitubercular preparations

Apparently from the table 6 there is observed dysfunction of membranes of hepatocytes after influence of anti-tubercular preparations and at the 18th days of research parameters of marker enzymes of a cytolysis and cholestasia of hepatocytes is increasing under the influence of RIP. Activity of AlAT, AAsT, LAP exceeds control data for 209%, 162% and in triple to comparison with intact groups, activity of AlPh increased to 62%, and activity of GGT increased to 6,4 times. The obtained data confirm literary data on increase of activity of LAP, AlAT, AAsT by influence with tuberculosis chemotherapy [202], it testifies to change of permeability of biological membranes of hepatocytes, about it is shown as decreasing resistance of membranes of hepatocytes (RMH) by 70% in comparison with control group.

Inclusion of PhPR in the treatment-and-preventetion mode with a dose 1,0ml/kg during 17 days will be reduced activity of AlAT(Alanin of amine transferase), AAsT(Amine aspartate transferase), LAP

which will be essential decreased their activity to 52%, 43,2%, 46,6% with comparison RIP group testifies. Activity of AlPh(ALKALINE PHOSPHATASE) decreased to 23,8%, and GGT(Gamma glutamit transpeptidaz) decreased to 4,1 times in comparison with uncured group of increasing RMG(Rezistention Membrane of Hepatocytes) was maded 126%. Using PhPR in a dose 2,0ml/kg of weight acutely decreased activity of AlAT, AAsT, LAP, AlPh and GGT to 65,6%, 63,6%, 65,3% , 39,1%, and 84% in comparison with uncured group, and decrease of the activity specified enzymes which are shown to 28,3%, 35,8%, 35,1% , 20% and 33,6% with the group which received PhPR in a dose of 1,0 ml/kg of weight. Resistance of membranes of hepatocytes increased more than triple and 44% reaches indicators of intact animals in

Thus, the conducted researches showed that under the influence of RIP in blood activity cytolytic and cholesterolical enzymes of a liver due to increase of permeability of biomembranes of hepatocytes, and inclusion of PhPR decrease reduces the increased level cytolytic and the cholesterolical enzymes in blood due to increase of resistance of membranes of hepatocytes. The maximum effects are noted after including PhPR in a dose of 2,0 ml/kg of body weight due to 17 days. Conclusion.

At influence of anti-tubercular preparations there is seen decreasing resistance of biomembranes of hepatocytes to 70%, due to activation of per oxidation of lipids and a depression of AOS.

Anti-radical action of PhPR depends on a dose, the most effective doses are at influence of tuberkulostatik of 2 ml/kg of weight due to 17 days treatment-and-preventetion actions of RDF-2 at intoxication drugs is caused by antioxidant action by influencing anti-tubercular preparations.

LITERATURE

1. Rmukhambetov D. D., Shaydarov M. Z., Gurtskaya G. M. Vegetable medicines at defeats of gepatobiliarny system//Sb. scientific works "Actual Problems of Pharmacology". - Astana. - 2002. - Page 131-139
2. Rochinsky VA. Phenolic antioxidants. Reactionary ability and efficiency. - Moscow, 1998.-110 pages. Marchishin See Efficiency of phenolic compounds of arnica at toxic damage of a liver//Farm. and current.- 1983. - No. 2. - Page 102-104
   1. 1. Ormanova L.N. Influence of a phytopreparation of a rhodiola pink and a ruvimina on hepatotoxic reactions at polychemotherapy of patients with tuberculosis of lungs//the Person and drugs. - Moscow.- 2006. - Page 230.